Microscope Instruction Manual Models # 4010, 4020 and 4030 Table of Contents Introduction.................................................................................. 1 Junior Microscope Model 4010........................................................ 2 Junior Dissecting Microscope Model #4020....................................... 4 Educational Microscope Model #4030.............................................. 6 Care and Maintenance.................................................................... 8 Introduction Congratulations on your purchase of a Celestron microscope. Celestron offers a complete line of quality microscopes ranging from beginner and educational microscopes to research level instruments. Your Celestron microscope is a precision optical instrument, made of the highest quality materials to ensure durability and long life. Your microscope is designed to give you a lifetime of pleasure with a minimal amount of maintenance. The first sections of this manual will help you become familiar with the various parts of your microscope and how they work together. Read carefully the sections on viewing specimens and focusing. Take special notice of all precautions regarding using the illuminator mirror (if applicable to your model) to focus sunlight and regulating the focuser travel so as not to damage specimen slides or the objective lens. The final section provides simple care and maintenance tips for you to follow to ensure that your microscope provides you with years of quality performance and enjoyment. 2 Junior Microscope ­ Model #4010 1 8 2 7 3 6 5 4 Figure 1 1 2 3 4 Eyepiece Objective Lens Specimen Stage Illumination Mirror 5 6 7 8 Specimen Holder Clips Focus Travel Safety Screw Stand Column Focus Adjustment Knob Standard Accessories: Your Celestron microscope (#4010) comes complete with the following standard accessories: · · · 10x Eyepiece 20x Objective lens (200x) Plastic Dust Cover Item #4010 Stage Focuser Objective Illuminator Eyepiece Specifications: Plain stage with paired clips Rack and Pinion; 30mm travel with focus stop Achromatic Objective 20x 42mm Plano-Convcave Mirror 10x; 18mm 2 Microscope Operation Using the Illuminator Mirror: Your Celestron microscope has a built-in Plano-concave illumination mirror which allows you to illuminate the specimen from the bottom. The mirror has a flat side and a concave side. The concave side will concentrate more light onto the specimen than the flat side. To illuminate a specimen: 1. If it is daylight, tilt the illumination mirror (4) and reflect indirect sunlight through the bottom of specimen stage (3). WARNING: Never use the mirror to focus direct sunlight through your microscope. Sunlight, if viewed directly can be very harmful and may permanently damage your eyes. 2. If viewing at night or indoors, away from sunlight, you can use a nearby desk or table lamp to reflect light onto a specimen. To control the amount of light reflecting onto the specimen: 1. Use the concave side of the illuminator mirror to focus more light on the slide. The concave side will concentrate more light onto your specimen but will focus a smaller beam of light, making it more sensitive to adjust. 2. If you are unable to adjust the mirrors enough to properly illuminate the specimen, you can also tilt the stand column (7) backwards to change the position of the mirror. Viewing a Specimen To view a specimen: 1. Carefully place a specimen slide under the specimen holder clips (5) and center the specimen directly over the hole in the stage (3). Your are know ready to focus and view the specimen, but first you must take some precautions so as not to damage the specimen slide or objective lens. Your microscope is equipped with a focus travel safety screw (6) which allows you to regulate the range of travel on the focuser. This assures that the objective lens will not accidentally come in contact with the specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focuser mechanism: 1. Raise the objective lens away from the specimen stage (3) by rotating the focuser knob (8) backwards. 2. Unthread the focus travel safety screw (6) about 2/3 of the way. 3. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to touch the objective lens to the slide). 4. Thread the focus travel safely screw (6) upward until it stops against the focuser mechanism. 5. Rotate the knurled locking nut upward to lock the safety screw in place. 6. Now, turn the focus adjustment knob (8) until the specimen comes into sharp focus. 3 Junior Dissecting Microscope ­ Model #4020 10 1 2 3 4 9 5 8 6 7 Figure 2 1 2 3 4 5 Monocular Head Pillar Stand Safety Screw Pillar Stand Pillar Stand Locking Bolt Focus Adjustment Knob 6 7 8 9 10 Base Stage Plate Specimen Slide Holder Clips Objective Lens Eyepiece Standard Accessories: Your Celestron microscope (#4020) comes complete with the following standard accessories: · · · 10x Eyepiece 4x Objective lens (40x) Plastic Dust Cover Item #4020 Optical Head Stage Focuser Nosepiece Objective Eyepiece Specifications: 45º inclined Monocular Head; Rotatable 60mm black/white stage plate and paired clips Rack and Pinion, Turret objective 4x 4x; Working Distance 58mm 10x; 18mm 4 Assembling Your Microscope Before you can view through your Celestron microscope, the monocular head assembly must be attached to the pillar stand (3). To attach the monocular head assembly: 1. Remove the safety screw from the top of the pillar stand (3). 2. Partially unthread the pillar stand locking bolt (4) located behind the focuser assembly. 3. Place the monocular head assembly onto the pillar stand (3) and slide it halfway down the stand. 4. Rotate the monocular head assembly until the objective lens (9) is directly over the center of the stage plate (7). 5. Tighten the pillar stand locking bolt (4) to hold the monocular head assembly in place. 6. Thread the safety screw (2) back onto the top of the pillar stand. Your microscope is now ready to view specimens. Microscope Operation To view a specimen: 1. Place the round black/white stage plate (7) into the opening on the base (6). 2. Carefully place a specimen slide under the specimen holder clips (8) and center the specimen directly in the middle of the stage plate. 3. Illuminate the specimen slide by using a nearby desk or table lamp to reflect light onto a specimen. Natural light can also be used if the microscope is located near a window. Tip: Experiment with different lighting for different specimens. When using downward lighting, the white side of the stage plate may wash out fine detail whereas the black side will increase contrast but may make the specimen less bright. Focusing: To focus on your specimen slide, turn the focus adjustment knob (5) until the specimen comes into focus. If there is not enough travel in the focuser mechanism to reach focus, it may be necessary to loosen the pillar stand locking bolt (4) and slightly raise or lower the entire binocular head assembly until the image becomes sharp. 5 Educational Microscope - Model #4030 1 2 12 3 4 11 5 10 9 8 7 6 Figure 3 1 2 3 4 5 6 Eyepiece Eyepiece Holder Objective Lens Rotator Objective Lens Specimen Stage Diaphragm Dial 7 8 9 10 11 12 Illuminator Mirror Specimen Holder Clips Focus Travel Safety Screw Stand Column Fine Focus Adjustment Knob Course Focus Adjustment Knob Standard Accessories: Your Celestron microscope (#4030) comes complete with the following standard accessories: · · · Huygens 10x eyepiece Huygens 12.5x eyepiece Plastic Dust Cover · · · 4x Objective lens 10x Objective lens 40x Objective lens Magnification Table Use the following table to determine the magnification of the different eyepiece/objective lens combination of your microscope. Eyepiece Objective Lens 4x 10x 10x 12.5x 40x 50x 100x 125x 40x 400x 500x 6 Item #4030 Objective Lens Nosepiece Stage Illuminator Diaphragm Focuser Specifications Achromatic Objective Triple nosepiece with click stop 120mm x 110mm, with paired clips 50mm Plano-Concave Disc diaphragm with five aperture sizes Course focus; Fine focus adjustments Assembling Your Microscope Before you can view through your Celestron microscope, the eyepiece and objective lenses must first be attached. Installing the Eyepiece: 1. Remove the 10x eyepieces from its container. 2. Slide the narrow end of the eyepiece barrel into the eyepiece holder at the top of the microscope. Installing the Objective Lenses: 1. Remove the three objective lenses from their individual containers. Unscrew the container lids from the threaded portion of the objective lenses. 2. Thread the end of the 4x objective lens into one of the holes on the objective lens rotator (3). It may be necessary to lower the specimen stage (5) by turning the coarse focus knob (12). 3. Now turn the objective rotator to the next opening and thread each of the remaining objective lenses into the remaining holes. Your microscope is now ready to view specimens. Microscope Operation Using the Illuminator Mirror: Your Celestron microscope has a built-in Plano-concave illumination mirror which allows you to illuminate the specimen from the bottom. The mirror has a flat side and a concave side. The concave side will concentrate more light onto the specimen than the flat side. To illuminate a specimen: 1. If it's daylight, tilt the illumination mirror (7) and reflect indirect sunlight through the bottom of specimen stage (5). WARNING: Never use the mirror to focus direct sunlight through your microscope. Sunlight, if viewed directly can be very harmful and may permanently damage your eyes. 2. If viewing at night or indoors, away from sunlight, you can use a nearby desk or table lamp to reflect light onto a specimen. To control the amount of light reflecting onto the specimen: 1. Use the concave side of the illuminator mirror to focus more light on your specimen. The concave side will concentrate more light onto your specimen but will focus a smaller beam of light, making it more sensitive to adjust. 7 2. The amount of light can also be controlled by rotating the five-aperture size diaphragm dial (6) underneath the specimen stage. 3. If you are unable to adjust the mirrors enough to properly illuminate the specimen, you can also tilt the stand column (10) backwards to change the position of the mirror. Viewing a Specimen To view a specimen: 2. Press downward on the back portion of the spring-loaded specimen holder clips (8) to raise the front of the clips off of the specimen stage (5). 3. Place a specimen slide under the specimen holder clips (8) and center the specimen directly over the hole in the center of the specimen stage (5). Your are know ready to focus and view the specimen, but first you must take some precautions so as not to damage the specimen slide or objective lens. Your microscope is equipped with a focus travel safety screw (9) which allows you to regulate the range travel on the focuser. This assures that the objective lenses will not accidentally come in contact with specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focuser mechanism: 7. Raise the objective lens away from the specimen stage (5) by rotating the course focuser knob (12) backwards. 8. Unthread the focus travel safety screw (9) about two-thirds of the way. 9. Turn the objective lens rotator (3) until the 40x lens is over the specimen. 10. Lower the objective lens over the slide until the tip of the lens is slightly above the specimen slide. (Be careful not to touch the objective lens to the slide). 11. Thread the focus travel safely screw (9) upward until it stops against the focuser mechanism. 12. Rotate the knurled locking nut upward to lock the safety screw in place. Focusing: Now that the specimen slide is placed directly under the objective lens, use the course focus knob (12) to focus on the specimen. · · · Always start with the lowest power (4x objective lens and 10x eyepiece) and switch to higher power by rotating the objective lens rotator (3). Be cautious not to let the objective lens touch the specimen slide when changing to higher power. Use the fine focus knob (11) to change the depth of field on the specimen you are observing. If you run out of downward travel before reaching sharp focus, you can unscrew the focus travel safety screw (9) to allow for more downward travel. Once again be careful not to let the objective lens touch the specimen slide. 8 Care and Maintenance Your Celestron microscope is a precision optical instrument and should be treated with care at all times. Follow these maintenance suggestions and your microscope will need very little maintenance throughout its lifetime. · · · · · · · · Always place the plastic cover over the microscope when not in use or when being stored. When moving your mic ...