Microscope Instruction Manual Models # 4040, 4050 and 4060 Table of Contents Introduction ................................................................................. Stereo Dissecting Microscope Model #4040 ..................................... Biological Microscope Model #4050 ................................................. Research Microscope Model #4060 .................................................. Care and Maintenance .................................................................... 1 2 5 8 12 Introduction Congratulations on your purchase of a Celestron microscope. Celestron offers a complete line of quality microscopes ranging from beginner and educational microscopes to research level instruments. Your Celestron microscope is a precision optical instrument, made of the highest quality materials to ensure durability and long life. Your microscope is designed to give you a lifetime of pleasure with a minimal amount of maintenance. The first sections of this manual will help you become familiar with the various parts of your microscope and how they work together. Read carefully the sections on viewing specimens and focusing. Take special notice of all precautions regarding using the illuminator mirror (if applicable to your model) to focus sunlight and regulating the focuser travel so as not to damage specimen slides or the objective lens. The final section provides simple care and maintenance tips for you to follow to ensure that your microscope provides you with years of quality performance and enjoyment. 2 Stereo Dissecting Microscope ­ Model #4040 1 2 13 3 4 12 5 11 10 9 6 7 8 Figure 1 1 2 3 4 5 6 7 Eyepiece Diopter Ring Binocular Head Binocular Head Tension Screw Objective Turret Slide Holder Clips Glass Stage Plate 8 9 10 11 12 13 Power Cord Illuminator Switch Top Light Illuminator Focus Adjustment Knob Pillar Stand Locking Bolt Pillar Stand Standard Accessories: Your Celestron microscope (#4040) comes complete with the following standard accessories: · · · Two 10x Eyepieces One 2x Objective lens (20x) One 4x Objective lens (40x) · · · Rubber Eyecups Black/White Stage Plate Plastic Dust Cover Item #4040 Optical Head Interpupillary distance Illuminator Stage Power Supply Nosepiece Objectives Specifications: 45º inclined Binocular Head; 360º Rotatable Adjustable from 55-75mm Top/Bottom built-in electric illuminator; Incandescent Bulb 94.5mm glass stage and black/white stage plate 110v Turret objective 2x/4x Achromatic objectives 2 Assembling Your Microscope Before you can view through your Celestron microscope, the binocular head assembly must be attached to the pillar stand (13) and the rubber eyecups must be placed over the eyepieces. To attach the binocular head assembly: 1. Remove the safety screw from the top of the pillar stand (13). 2. Partially unthread the pillar stand locking bolt (12) located behind the focuser assembly. 3. Place the binocular head assembly onto the pillar stand (13) and slide it half way down the stand. Be careful not to put too much tension on the cord that goes from the microscope base to the top light illuminator (10). 4. Rotate the binocular head assembly until the objective turret (5) is directly over the center of the stage (7). 5. Tighten the pillar stand locking bolt (12) to hold the binocular head assembly in place. 6. Thread the safety screw back onto the top of the pillar stand. To Attach the Rubber Eyecups: 1. Place the rubber eyecups over the eyepieces with the rubber lips facing up. 2. Push the eyecups down over the tops of the eyepieces. 3. Rotate the eyepieces so that the rubber lips are facing out to the sides. Your microscope is now ready to view specimens. Microscope Operation Powering the illuminator: Your Celestron microscope has a built-in, 3-setting illuminator powered by 110v AC power. To power the illuminator: 1. 2. 3. 4. Plug the power cord (8) into a wall outlet. Turn the illuminator switch (9) to the "I" setting to turn on the bottom illuminator bulb. Turn the illuminator switch (9) to the "II" setting to turn on the top illuminator bulb. Turn the illuminator switch (9) to the "III" setting to turn on both the top and bottom illuminator bulbs. Viewing a Specimen To view a specimen: 1. Place the round glass stage plate (7) into the opening above the illuminator. When viewing a specimen using top lighting only, you may wish to replace the glass stage plate with the black/white stage plate. Tip: Specimens of different size and thickness will require different levels of illumination. Experiment with different lighting for each specimen. When using top lighting, the white side of the stage plate may wash out fine detail where as the black side will increase contrast but may make the specimen less bright. 2. Place a specimen slide under the specimen holder clips (6) and center the specimen directly in the center of the stage. 3 Focusing: To achieve the best focus with your microscope, not only can you make adjustments with the focus adjustment knobs, but you can also adjust the left diopter ring as well as the interpupillary distance between the eyepieces. To achieve sharpest focus: 1. Rotate the objective turret (5) to the 2x position . (Always begin by viewing a specimen at low power, then switch to high power when needed). 2. Turn the focus adjustment knob (11) until the specimen comes into focus. If there is not enough travel in the focuser mechanism to reach focus, it may be necessary to loosen the pillar stand locking bolt (12) and slightly raise or lower the entire binocular head assembly until the image becomes sharp. 3. Next, while looking through the eyepieces, adjust the interpupillary distance by sliding both eyepieces side to side until the whole field of view can be observed through both eyes at the same time. 4. Finally, close your right eye and turn the left eyepiece diopter ring until the image is in sharp focus through the eyepiece. If higher power is desired, rotate the objective turret (5) to the 4x position and readjust the focus knobs (11) until the specimen comes into focus. 4 Biological Microscope - Model #4050 1 2 15 3 14 4 5 6 13 7 12 8 11 9 10 1 2 3 4 5 6 7 8 Eyepiece Monocular Head Objective Lens Rotator Objective Lens Condenser Specimen Stage Diaphragm Dial Illuminator 9 10 11 12 13 14 15 Power Switch Fine Focus Adjustment Knob Power Cord Course Focus Adjustment Knob Specimen Holder Clips Focus Travel Safety Screw Stand Column Standard Accessories: Your Celestron microscope (#4050) comes complete with the following standard accessories: · · 10x eyepiece with pointer Plastic Dust Cover · · · 4x Objective lens (40x) 10x Objective lens (100x) 40x Objective lens (400x) Item #4050 Objective Lens Nosepiece Stage Illuminator Diaphragm Head Focuser 5 Specifications Achromatic Objective Triple nosepiece with click stop 120mm x 110mm, with paired clips Built-in electric illuminator; 20W tungsten bulb Disc diaphragm with five aperture sizes Monocular head, 45º incline, 360º rotatable Course focus; Fine focus adjustments Assembling Your Microscope Before you can view through your Celestron microscope, the objective lenses must first be attached. Installing the Objective Lenses: 1. Remove the three objective lenses from their individual containers. Unscrew the container lids from the threaded portion of the objective lens. 2. Thread the end of the 4x objective lens into one of the holes on the objective lens rotator (3). It may be necessary to lower the specimen stage (6) by turning the coarse focus knob (12). 3. Now turn the objective rotator to the next opening and thread each of the remaining objective lenses into the remaining holes. Your microscope is now ready to view specimens. Microscope Operation Powering the illuminator: Your Celestron microscope has a built-in illuminator powered by 110v AC power. To power the illuminator: 5. Plug the power cord (11) into a wall outlet. 6. Turn the power switch (9) to the "on" position. To regulate the amount of light shining on the specimen, rotate the diaphragm dial (7). The diaphragm dial has five openings of different sizes which allow different amounts of light to pass from the illuminator. Viewing a Specimen To view a specimen: 3. Press downward on the back portion of the spring-loaded specimen holder clips (13) to raise the front of the clips off of the specimen stage (6). 4. Place a specimen slide under the specimen holder clips (13) and center the specimen directly over the condenser lens . You are now ready to focus and view the specimen, but first you must take some precautions so as not to damage the specimen slide or objective lens. Your microscope is equipped with a focus travel safety screw (14) which allows you to regulate the range of travel on the focuser. This assures that the objective lenses will not accidentally come in contact with specimen slide, breaking the slide or scratching the objective lens. To adjust the travel on the focuser mechanism: 1. 2. 3. 4. Lower the specimen stage by rotating the course focus adjustment knobs (12). Partially unscrew the focus travel safely screw (14) so that about 1 inch is sticking up. Turn the objective lens rotator (3) until the 40x lens is directly over the light condenser (5). Use the focus knobs (12) to raise the stage (6) until the tip of the lens is slightly above the specimen slide. (Be careful not to touch the slide to the objective lens). 5. Thread the focus travel safely screw (14) down until it stops against the focuser mechanism. 6. Rotate the knurled locking nut downward to lock the safety screw in place. 6 Focusing: Now that the specimen slide is placed directly under the objective lens, use the course focus knob (12) to focus on the specimen. · Always start with the lowest power (4x objective lens) and switch to higher power by rotating the objective lens rotator (3). Be cautious not to let the objective lens touch the specimen slide when changing to higher power. · Use the fine focus knob (10) to change the depth of field on the specimen you are observing. · If you run out of upward travel before reaching sharp focus, you can unscrew the focus travel safety screw (14) to allow for more upward travel. Once again be careful not to let the objective lens touch the specimen slide. Replacing the illuminator lamp: 1. Raise the specimen stage (6) to its highest position by rotating the course focus adjustment knob (12). 2. Unscrew the black metal illuminator housing (8) and lift it straight up over the lightbulb. 3. Turn the lightbulb counterclockwise while gently pushing down. 4. Replace with a new 20W tungsten bulb. 5. Slide the illuminator housing back over the lightbulb and thread into place. 7 Research Microscope ­ Model #4060 115 1 2 3 21 20 19 18 17 16 15 14 4 5 6 7 8 9 10 13 12 11 Figure 3 1 2 3 4 5 6 7 8 9 10 11 Eyepiece Eyepiece Diopter Eyepiece Housing Locking Screw Stand Column Specimen Holder Focuser Lock Coarse Focus Handwheel Fine Focus Handwheel Transverse Specimen Mover Handwheel Brightness Control Wheel 12 13 14 15 16 17 18 19 20 21 Longitudal Specimen Mover Handwheel Power Switch Illuminator Condenser Adjustment Handwheel Iris Diaphragm Condenser Specimen Stage Specimen Holder Clamp Lever Objective Objective Changer Standard Accessories Your Celestron microscope (#4060) comes complete with the following standard accessories: · · · · · Two 10x Eyepieces Two 15x Eyepieces One 4x Objective lens One 10x Objective lens One 40x Objective lens · · · · One 100x Objective lens Three Color Filters (Red, Blue ,Green) Plastic Dust Cover Cedar Wood Oil 8 Item #4060 Optical Head Interpupillary distance Illuminator Stage Size Working Range Condenser Power Supply Nosepiece Objectives Specifications: 45º inclined Binocular Head; 360º Rotatable Adjustable from 55-75mm Built-in, electric illuminator; Halogen bulb 6v/20w Built-in, Mechanical 123mm x 153mm 70mm x 40mm Abbe N.A. 1.25 condenser, and iris diaphragm 110v Quadruple nosepiece Achromatic objectives Magnification Table Use the following table to determine the magnification of the different eyepiece/objective lens combination of your microscope. Objective Lens 10x 100x 150x Eyepiece 4x 10x 15x 40x 60x 40x 400x 600x 100x 1000x 1500x Assembling Your Microscope Before you can view through your Celestron microscope, the eyepiece housing, eyepieces and objective lenses must first be attached. To attach the eyepiece housing: 1. Remove the microscope body from its packaging by lifting it by the stand column (5). 2. Unthread the locking screw (4) located on the side of the microscope body just above the objective lens changer (21). 3. Place the round angled end of the eyepiece housing (3) inside the circular opening at the top of the microscope body. The round eyepiece housing adapter is slightly angled and should slide under the two metal tabs on the inside of the microscope body opening. 4. Secure the eyepiece ...