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User manual FLUORESCENCE FM-PCI-PC-DAQ2.7

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Manual abstract: user guide FLUORESCENCE FM-PCI-PC-DAQ2.7

Detailed instructions for use are in the User's Guide.

FluorMeasureTM Version 2.7 Acquisition Software User Manual for the PC-DAQ Controller C&L Instruments, Inc. 314 Scout Lane Hummelstown, PA 17036 Phone: 717-564-9491 Fax: 802-609-1713 E-mail: support@fluorescence.com Web site: www.fluorescence.com Table of Contents Table of Contents List of Figures.........................................................................................................................................5 Chapter 1: About FluorMeasureTM ......................................................................................................6 Program Description........................................................................................................................................... 6 System Requirements ......................................................................................................................................... 6 Technical Support............................................................................................................................................... 6 About this Manual .............................................................................................................................................. 7 What's Covered ............................................................................................................................................. 7 Obtaining Current Documentation ................................................................................................................ 7 Chapter 2: Software and Hardware Installation ................................................................................8 Overview ............................................................................................................................................................ 8 Installing the Hardware ...................................................................................................................................... 8 Installing the PC-DAQ Controller Card........................................................................................................ 8 Installing the Data Acquisition Software ....................................................................................................... 8 Chapter 3: Fundamentals of FluorMeasureTM ..................................................................................10 Overview ...............................................................................................................................................10 Filter Wheels .................................................................................................................................................... 10 Filter Wheel Type ........................................................................................................................................ 10 Filter Wheel Operation Modes .................................................................................................................... 10 Data Acquisition Modes ................................................................................................................................... 11 Shutters............................................................................................................................................................. 11 Fluorescence and Photon Counting .................................................................................................................. 11 Acquiring Single Sample Measurements and Data Averaging......................................................................... 12 Fluorescence and Fluorescence Ratios ............................................................................................................. 12 Excitation Light Intensity and The Neutral Density Wheel.............................................................................. 13 Measuring Fluorescence by Balancing Factors ................................................................................................ 13 Excitation Lamp Intensity ............................................................................................................................ 13 Specification of Excitation and Emission Interference Filters..................................................................... 14 Sampling Time Setting ................................................................................................................................. 14 Acquisition of Analog Data.............................................................................................................................. 14 Analog Output .................................................................................................................................................. 14 Time-sharing .................................................................................................................................................... 15 Chapter 4: Getting Started..................................................................................................................16 Overview .......................................................................................................................................................... 16 The Data Acquisition Process........................................................................................................................... 16 Basic Steps in Data Acquisition ....................................................................................................................... 16 Chapter 5: Command Reference ........................................................................................................17 Overview .......................................................................................................................................................... 17 Main Program Window and Child Windows ................................................................................................... 17 Drop-down Menu Bar....................................................................................................................................... 18 File Menu ......................................................................................................................................................... 19 File / New..................................................................................................................................................... 19 File / Open ................................................................................................................................................... 19 File / Close................................................................................................................................................... 19 File / Save .................................................................................................................................................... 20 File / Save As............................................................................................................................................... 20 File / 1. [file name] (etc.)............................................................................................................................. 20 File / Exit ..................................................................................................................................................... 20 Options Menu ................................................................................................................................................... 20 FluorMeasure (version 2.7) Data Acquisition Software User Manual For the PC-DAQ Controller TM Page 2 Table of Contents Options / Setup... ......................................................................................................................................... 21 Options / WDF Manager............................................................................................................................. 34 Experiment Menu ............................................................................................................................................. 34 Acquisition Menu ............................................................................................................................................. 35 Shutter Menu .................................................................................................................................................... 35 Monitor Menu................................................................................................................................................... 35 Monitor / New Monitor Window .................................................................................................................. 35 Monitor / Cascade ....................................................................................................................................... 36 Monitor / Tile............................................................................................................................................... 36 Monitor / Arrange Icons .............................................................................................................................. 36 Monitor / Zoom Tool.................................................................................................................................... 36 Monitor / Unzoom Tool................................................................................................................................ 36 Help Menu ........................................................................................................................................................ 36 Help / About FluorMeasure......................................................................................................................... 36 Help / Shortcut Keys .................................................................................................................................... 37 Chapter 6: Using the Icons in the Toolbar.........................................................................................38 Overview .......................................................................................................................................................... 38 The Icons .......................................................................................................................................................... 38 Chapter 7: Using Keyboard Shortcuts...............................................................................................39 Chapter 8: The Status Bar ..................................................................................................................40 Chapter 9: Acquiring Data..................................................................................................................41 Overview .......................................................................................................................................................... 41 Data and Configuration Files............................................................................................................................ 41 Data Files .................................................................................................................................................... 41 Configuration Files...................................................................................................................................... 41 Using the Setup Options ................................................................................................................................... 41 Data Acquisition Modes............................................................................................................................... 42 Using Run......................................................................................................................................................... 43 Using the Neutral Density Wheel and Avoiding Peak Pile Up......................................................................... 44 The Neutral Density Wheel .......................................................................................................................... 44 Avoiding Peak Pile Up................................................................................................................................. 44 Using the Shutters and Avoiding Shoot Through ............................................................................................. 45 The Excitation and Emission Shutters ......................................................................................................... 45 Avoiding Shoot Through .............................................................. ...
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