HINTS FOR ACCURATE MEASUREMENTS 1. Use the pipette in a vertical position whenever possible. 2. Operate slowly and smoothly. Aspiration and dispensing should been carried-out with the same speed. 3. The tip should be immersed in the sample liquid the minimum necessary (2-4 mm). Greater depths may cause inaccurate measures. 4. Change the tip whenever a different kind of sample is to be aspired or when droplets remain on the inside wall of the tip from the previous pipetting operation. 5. Work always with samples at room temperature. Instruction Guide Fixed-Volume Pipette HI 731340 HI 731341 HI 731342 http://www.hannainst.com PIPETTE MAINTENANCE 1. Liquid should never enter the pipette shaft. To avoid this: · never use pipette without a tip · do not invert pipette upside down or lay it flat when there is a liquid in the tip · press and release the push button slowly and smoothly. 2. When pipetting concentrated acids or alkalis or corrosive solutions, pay particular attention to avoid contact of the liquid with the pipette shaft, or it could be seriously damaged. Extensive contact with corrosive fumes may result in premature ageing of internal components of the pipette. 3. After use, store the pipette in its case or in a clean safe place. Dear Customer, Thank you for choosing a Hanna Instruments product. This instruction guide will provide you with the necessary information for the correct use of the pipette. Please read it carefully before using the pipette. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that no damage has occurred during shipment. If there is any damage, notify your Dealer. Each pipette is supplied complete with: · Two tips · Instruction Guide · Rigid carrying case Note: Keep all packing material until you did verify that the pipette works correctly. Any defective item must be returned in its original packing. ACCESSORIES HI 731350 HI 731351 HI 731352 tips for 200 µL automatic pipette (25 pcs) tips for 1000 µL automatic pipette (25 pcs) tips for 2000 µL automatic pipette (4 pcs) GENERAL DESCRIPTION HI 731340/1/2 is an air-displacement volumetric pipette designed to measure and transfer fixed volumes of liquids that needs to be precisely dosed. The liquid is aspirated into deposable tips. The use of disposable tips eliminate cross contamination of the liquid samples. To discard used tips, the pipette is equipped with a built-in tip ejector to protect the user from contaminated tips. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA. Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice. ISTMICPIPR1 05/03 http://www.hannainst.com Hanna Instruments Srl V.le delle Industrie 12/A 35010 Villafranca (Padova) ITALY Description: Pushbutton second stop first stop OPERATION Tip ejector button Pipette volume: 200, 1000 or 2000 µL 1. Mounting a tip: Place a new tip on the pipette by pressing and gently rotating the shaft into the tip. The tip will fit firmly the shaft. Note: Never use the pipette without a tip. 2. Aspiration: · Press the pushbutton to the first stop (fig. A). Holding the pipette vertically, immerse the tip into the sample liquid to the depth of 2-4 mm. · Release the pushbutton slowly and smoothly (3-6 seconds). Pause (1-2 seconds) to allow the full volume of liquid to fill up the tip (longer for high density or viscous liquids) (fig. B). · Withdraw the pipette out of the liquid. If any liquid remains on the outside of the tip, wipe it away carefully with a lint-free cloth (take care not to touch the tip orifice). 3. Dispensing: · Holding the pipette vertically, position the tip end against the sidewall of the receiving cuvet at a 40-45° angle. Expel the liquid by pressing slowly the pushbutton; pass by the first stop to the second stop (bottom of the stroke length). Wait 13 seconds to empty completely the tip (longer for viscous liquids) (fig. C). · Still holding the pushbutton depressed, withdraw the pipette sliding it along the wall of the vessel. Then release the pushbutton. · Discard the tip to waste container by pressing the tip ejector button (fig. D). · The pipette is ready for the next pipetting cycle. To prevent cross contamination use a new tip whenever a different kind of sample is to be aspirated. · The pipette tip may be used several times, rinse the tip immediately after use. A dirty tip may cause cross contamination or wrong sample volumes may be transferred. Body Shaft Tip ejector arm Disposable tip Operation: A B C D Technical data: Pipette code HI 731340 HI 731341 HI 731342 Tip code HI 731350 HI 731351 HI 731352 Volume (µL) 200 1000 2000 Accuracy Precision (%) (%) ±1.5 ±1.2 ±1.0 0.8 0.5 0.3 ...