Instruction Manual HI 83225 Grow Master for Nutrient Analyses MAN83225R1 04/05 w w w . h a n n a i n s t . c o m www.hannainst.com This Instrument is in Compliance with the CE Directives Dear Customer, Thank you for choosing a Hanna product. Please read this instruction manual carefully before using the meter. This manual will provide you with the necessary information for the correct use of the instrument. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. These instruments are in compliance with directives. HANNA LITERATURE Hanna publishes a wide range of catalogs and handbooks for an equally wide range of applications. The reference literature currently covers areas such as: TABLE OF CONTENTS PRELIMINARY EXAMINATION ....... 3 GENERAL DESCRIPTION ............... 3 SPECIFICATIONS ......................... 4 PRINCIPLE OF OPERATION .......... 4 FUNCTIONAL DESCRIPTION .......... 6 GUIDE TO DISPLAY CODES .......... 7 TIPS FOR AN ACCURATE MEASUREMENT ........................ 10 PARAMETERS REFERENCE TABLE 11 OPERATIONAL GUIDE ................ 12 ABBREVIATIONS ....................... 13 INTRODUCTION ........................ 14 SAMPLE PREPARATION .............. 19 AMMONIA LR ........................... 23 AMMONIA MR .......................... 25 AMMONIA HR ........................... 27 CALCIUM .................................. 29 MAGNESIUM ............................ 31 NITRATE LR .............................. 33 NITRATE MR ............................. 35 NITRATE HR ............................. 37 PHOSPHORUS LR ..................... 39 PHOSPHORUS MR .................... 41 PHOSPHORUS HR ..................... 43 POTASSIUM LR ......................... 45 POTASSIUM MR ........................ 47 POTASSIUM HR ........................ 49 POTASSIUM UHR ...................... 50 SULFATE .................................. 51 INTERFACE WITH PC ................. 53 STANDARD METHODS ............... 55 BATTERY REPLACEMENT ............ 55 ACCESSORIES ........................... 56 WARRANTY ............................... 57 CE DECLARATION OF CONFORMITY ....................... 58 HANNA LITERATURE .................. 59 · · · · · · · Water Treatment Process Swimming Pools Agriculture Food Laboratory Thermometry and many others. New reference material is constantly being added to the library. For these and other catalogs, handbooks and leaflets contact your dealer or the Hanna Customer Service Center nearest to you. To find the Hanna Office in your vicinity, check our home page at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA. 2 59 CE DECLARATION OF CONFORMITY PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipment. If there is any damage, notify your Dealer. Each HI 83225 Meter is supplied complete with: · Four Sample Cuvets and Caps · One Transport Cap · Two 9 V Battery · 12 VDC Transformer (HI 710005 or HI 710006) Note: Save all packing material until you are sure that the instrument functions correctly. Any defective item must be returned in its original packing with the supplied accessories. GENERAL DESCRIPTION HI 83000 Series is a line of 18 different bench, microprocessor-based photometers that measure more than 50 parameters in water and wastewater. These multipurpose meters are manufactured to measure the most important parameters of the application they have been especially designed for: HI 83099 Laboratories, with COD HI 83200 Laboratories HI 83203 Aquaculture HI 83205 Boilers & Cooling Towers HI 83206 Environmental Testing HI 83207 Industrial Wastewater HI 83208 Water Conditioning HI 83209 Education HI 83210 Pulp & Paper Mills HI 83211 Chemical Manufacturers HI 83212 Power Plant Utilities HI 83213 Municipal Wastewater HI 83214 Wastewater Treatment Applic. HI 83215 Nutrient Analyses HI 83216 Pool & Spa Applic. HI 83218 Environmental Applic. HI 83225 Nutrient Analyses HI 83226 Pool & Spa Applic. All meters use an exclusive positive-locking system to ensure that the cuvet is in the same position every time it is placed into the measurement cell. The reagents are in liquid or powder form and are supplied in bottles or in packets. The amount of reagent is precisely dosed to ensure the maximum repeatability. Display codes aid the user in routine operations. The meters have an auto-shut off feature, turning the unit off after 10 minutes of non-use. The HI 83000 Series can be connected to a personal computer via the HI 920010 three wire RS 232 cable. The HI 92000 Hanna Windows® Compatible Software aids the user to manage all test data. Recommendations for Users Before using these products, make sure that they are entirely suitable for the environment in which they are used. Operation of these instruments in residential area could cause unacceptable interferences to radio and TV equipments, requiring the operator to take all necessary steps to correct interferences. Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance. To avoid damages or burns, do not perform any measurement in microwave ovens. 58 3 SPECIFICATIONS Tungsten lamp with narrow band interference filter Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing Power Supply 2x 9 V batteries / 12 to 20 VDC through voltage adapter Auto-Shut off After 10' of non-use Dimensions 230 x 165 x 70 mm (9.0 x 6.5 x 2.8") Weight 640 g (22.6 oz.) For specifications related to each single parameter (e.g. range, accuracy, etc.), refer to the related measurement section. Light Source WARRANTY All Hanna Instruments meters are warranted for two years against defects in workmanship and materials when used for their intended purpose and maintained according to instructions. This warranty is limited to repair or replacement free of charge. Damages due to accidents, misuse, tampering or lack of prescribed maintenance are not covered. If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection. To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase. Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice. PRINCIPLE OF OPERATION Absorption of Light is a typical phenomenon of interaction between Electromagnetic Radiation and Matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physicochemical characteristics of substance according to the Lambert-Beer Law: -log I/Io = c d or A = c d Where: -log I/Io= Absorbance (A) Io = intensity of incident light beam I = intensity of light beam after absorption = molar extinction coefficient at wavelength c = molar concentration of the substance d = optical path through the substance Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known. Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents. 4 57 ACCESSORIES REAGENT SETS HI 93706-01 HI 93706-03 HI 93715-01 HI 93715-03 HI 93728-01 HI 93728-03 HI 93750-01 HI 93750-03 HI 93751-01 HI 93751-03 HI 937520-01 HI 937520-03 HI 937521-01 HI 937521-03 100 Phosphorus tests 300 Phosphorus tests 100 Ammonia tests 300 Ammonia tests 100 Nitrate tests 300 Nitrate tests 100 Potassium tests 300 Potassium tests 100 Sulfate tests 300 Sulfate tests 50 Magnesium tests 150 Magnesium tests 50 Calcium tests 150 Calcium tests Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements. The optical system of Hanna's HI 83000 multiparameter photometers is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results. Four measuring channels (at four different wavelengths) allow a wide range of tests. OTHER ACCESSORIES HI 721310 9V battery (10 pcs) HI 731318 Tissue for wiping cuvets (4 pcs) HI 731321 Glass cuvets (4 pcs) HI 731325N New cap for cuvet (4 pcs) HI 740034 Cap for 100 mL beaker (6 pcs) HI 740036 100 mL plastic beaker (6 pcs) HI 740157 Plastic refilling pipette (20 pcs) HI 92000 Windows® Compatible Software HI 920010 PC connection cable HI 93703-50 Cuvets cleaning solution (230 mL) HI 740223 170 mL plastic beaker HI 740224 170 mL plastic beakers (12 pcs) HI 740225 60 mL graduated syringe HI 740226 5 mL graduated syringe HI 740227 Filter assembly HI 740228 Filter discs (25 pcs) HI 740229 100 mL Polypropylene cylinder HI 740230 230 mL Demineralized water HI 93703-54 Dried resin (100 g) HI 93703-55 Activated Carbon (50 pcs) HI 83000 Block diagram (optical layout) A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvet. The optical path is fixed by the diameter of the cuvet. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity Io or I. The photoelectric cell collects the radiation I that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range. The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD. The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed. The cuvet has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvets are optically identical to provide the same measurement conditions. Whenever possible use the same cuvet for both. It is also necessary that the surface of the cuvet is clean and not scratched. This is to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvet walls with hands. Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases, it is necessary to close the cuvet to prevent any contamination. 56 5 FUNCTIONAL DESCRIPTION FRONT PANEL STANDARD METHODS Description Ammonia, LR Ammonia, MR Ammonia, HR Nitrate, LR Nitrate, MR Nitrate, HR Phosphorus, LR Phosphorus, MR Phosphorus, HR Potassium, LR Potassium, MR Potassium, HR Calcium, Magnesium, HR Sulfate Program 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Range 0.0 to 10.0 mg / L 0.0 to 50.0 mg / L 0 to 100 mg/L 0.0 to 30.0 mg / L 0 to 150 mg/L 0 to 300 mg/L 0.0 to 10.0 mg/L 0.0 to 50.0 mg/L 0 to 100 mg/L 0.0 to 20.0 mg/L 10 to 100 mg / L 20 to 200 mg / L 0 to 400 mg/L 0 to 150 mg/L 0 to 150 mg/L Method Nessler Nessler Nessler Cadmium Reduction Cadmium Reduction Cadmium Reduction Aminoacid Aminoacid Aminoacid Turbidimetric Turbidimetric Turbidimetric Oxalate Calmagite Turbidimetric Cuvet Holder Dual Level Liquid Crystal Display Programs List READ DIRECT, to perform measurement immediately TIMER, to perform measurements after a preprogrammed countdown 6) ZERO, to zero the meter prior to measurement 7) Program and , to select the desired parameter 8) ON/OFF, to turn the meter on and off 1) 2) 3) 4) 5) BATTERY REPLACEMENT Besides to the 12 VDC supply, this meter can also be fed by two 9V batteries. To prolong battery life, switch your meter off after use. However, the meter has an auto-shut off feature that will turn itself off after 10 minutes of non-use. A blinking "LOBAT" on the display, while a measurement is in progress, indicates low voltage and the batteries should be replaced. If the batteries are not replaced immediately, in order to prevent erroneous readings due to low voltage, "-BA-" is displayed soon afterwards. At this point the batteries must be changed. Battery replacement must only take place in a non-hazardous area using two 9V alkaline batteries. Remove the battery cover ...