SALES AND TECHNICAL SERVICE CONTACTS Australia: Tel. (03) 9769.0666 · Fax (03) 9769.0699 Instruction Manual China: Tel. (10) 88570068 · Fax (10) 88570060 Egypt: Tel. & Fax (02) 2758.683 Germany: Tel. (07851) 9129-0 · Fax (07851) 9129-99 FREE & TOTAL SULPHUR DIOXIDE MINITITRATOR for wine analysis HI 84100 Greece: Tel. (210) 823.5192 · Fax (210) 884.0210 Indonesia: Tel. (21) 4584.2941 · Fax (21) 4584.2942 Japan: Tel. (03) 3258.9565 · Fax (03) 3258.9567 Korea: Tel. (02) 2278.5147 · Fax (02) 2264.1729 Malaysia: Tel. (603) 5638.9940 · Fax (603) 5638.9829 Singapore: Tel. 6296.7118 · Fax 6291.6906 South Africa: Tel. (011) 615.6076 · Fax (011) 615.8582 Taiwan: Tel. 886.2.2739.3014 · Fax 886.2.2739.2983 Thailand: Tel. 66.2619.0708 · Fax 66.2619.0061 MAN84100 09/05 United Kingdom: Tel. (01525) 850.855 · Fax (01525) 853.668 USA: Tel. (401) 765.7500 · Fax (401) 765.7575 For e-mail contacts and a complete list of Sales and Technical offices, please see www.hannainst.com. w w w. h a n n a i n s t . c o m 20 1 Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. This instrument is in compliance with directives. WARRANTY HI 84100 is warranted for two years against defects in workmanship and materials when used for its intended purpose and maintained according to the instructions. This warranty is limited to repair or replacement free of charge. Damage due to accident, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your dealer. If under warranty, report the model number, date of purchase, serial number and the nature of the failure. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization Number from the Customer Service Department and then send it with shipment costs prepaid. When shipping any instrument, make sure it is properly packaged for complete protection. To validate your warranty, fill out and return the enclosed warranty card within 14 days from the date of purchase. TABLE OF CONTENTS PRELIMINARY EXAMINATION................................................................................................. 3 GENERAL DESCRIPTION ........................................................................................................ 3 SPECIFICATIONS ................................................................................................................... 5 PRINCIPLE OF OPERATION .................................................................................................... 6 FUNCTIONAL DESCRIPTION ................................................................................................... 7 START UP ............................................................................................................................ 9 GUIDE TO DISPLAY CODES ................................................................................................... 10 TIPS FOR AN ACCURATE MEASUREMENT ............................................................................. 12 FREE SO2 MEASUREMENT PROCEDURE................................................................................ 12 TOTAL SO2 MEASUREMENT PROCEDURE .............................................................................. 13 PUMP CALIBRATION PROCEDURE ........................................................................................ 15 PUMP TUBE REPLACEMENT ................................................................................................ 15 FUSE REPLACEMENT .......................................................................................................... 16 ELECTRODE CONDITIONING AND MAINTENANCE .................................................................. 16 ACCESSORIES ..................................................................................................................... 18 WARRANTY ........................................................................................................................ 19 WARRANTY All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA. 2 Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice. 19 ACCESSORIES REAGENT SETS HI 70300L HI 70635L HI 70636L HI 7082 HI 84100-50 HI 84100-51 HI 84100-52 HI 84100-53 HI 84100-54 HI 84100-55 Electrode storage solution (500 mL) Cleaning solution for wine deposits (500 mL) Cleaning solution for wine stains (500 mL) Electrode filling solution (4 X 30 mL) Titrant solution (100 mL) Alkaline reagent (500 mL) Acid reagent for Total SO2 determination (500 mL) Acid reagent for Free SO2 determination (500 mL) Stabilizer reagent (25 pcs.) Calibration standard (500 mL) PRELIMINARY EXAMINATION Please examine this product carefully. Make sure that the instrument is not damaged. If any damage occured during shipment, please notify your Dealer. Each HI 84100 minititrator is supplied complete with: · Reagents set for 20 titrations · Two 50 mL beakers · Two 20 mL beakers · Scissors · Tubes set with cap · ORP probe · Stir bar · Power cable · One 30 mL bottle of Refill Solution · One 1 mL syringe · Two sachets of cleaning solution for wine deposits · Two sachets of cleaning solution for wine stains · Instruction manual Note:Save all packing material until you are sure that the instrument works correctly. Any defective item must be returned in its original packing. OTHER ACCESSORIES HI 3148B /50 ORP probe with 50 cm cable HI 70483T Tube set with cap for titrant bottle and tip HI 731316 Stir bar (5 pcs.) HI 740036P Beaker 50 mL (10 pcs.) HI 740037P Beaker 20 mL (10 pcs.) HI 740198 Power cable GENERAL DESCRIPTION The HI 84100 is a low-cost, easy to use, microprocessor-based automatic titrator that benefits from Hanna's years of experience as a manufacturer of analytical instruments. It has a simple and reliable peristaltic pump that ensure high dosing repeatability. By performing pump calibration with the provided Hanna standards, the instrument accuracy is assured. The instrument comes with a pre-programmed analysis method designed for Free and Total Sulphur Dioxide measurements on wine samples. The instrument has a powerful and effective built-in algorithm to analyze the shape of the electrode response and to determine the reaction completion. This algorithm automatize the analysis, makes all the necessary calculations and assures a simple and effective interface for the user. By simply pressing the START STOP button, the instrument will automatically make the titration up to the equivalence point. The result is immediately displayed in convenient units, then the instrument is ready for another titration. 3 Recommendations for Users Before using this product, make sure that it is entirely suitable for your specific application and for the environment in which it is used. Operation of this instrument may cause unacceptable interferences to other electronic equipments, this requiring the operator to take all necessary steps to correct interferences. Any variation introduced by the user to the supplied equipment may degrade the instrument EMC performance. To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the instrument safety do not use or store the instrument in hazardous environments. 18 SIGNIFICANCE OF USE An important reason for adding SO2 is to avoid oxidation. When there is oxygen around, SO2 itself becomes oxidized before phenol compounds in the wine , and so acts as an oxygen scavenger. Also SO2 suppresses the activity of enzymes that cause browning and other problems. What is really protecting your wine is molecular SO2. When you add SO2, depending of circumstances, some of it immediately becomes bound. The relationship between the amount of added SO2 and the amount of SO2 remaining free is complex. It is clear, however, that it is largely governed by the total SO2 content of the wine. The rate of binding decreases as the free SO2 concentration increases. The exact relationship between free and bound (total - free) SO2 will vary from wine to wine. Below 30-60 ppm, 33% to 50% of SO2 addition becomes bounded. What remains is called "free" and it is divided in two parts. The larger, and relatively ineffective free part is "bisulphite" (HSO3-). The smaller part of the free is the active molecular SO2. The amount of molecular SO2 in your wine depends both on the level of free SO2 present as well as pH. For instance, at pH 3.2, the amount of free SO2 for 0.8 ppm molecular SO2 is 22 ppm. At pH 3.5, you will need 43 ppm free - essentially double. Free SO2 concentration (ppm) for 0.8 ppm molecular SO2: pH Free SO2 3.0 14 3.1 18 3.2 22 3.3 28 3.4 35 3.5 44 3.6 55 3.7 69 3.8 87 3.9 109 For faster response, unscrew the fill hole screw during measurements. STORAGE PROCEDURE To minimize clogging and assure a quick response time, the glass bulb and the junction of the electrode should be kept moist and not allowed to dry out. Replace the solution in the protective cap with a few drops of HI 70300 Storage Solution or, in its absence, Filling Solution (HI 7082). Follow the Preparation Procedure before taking measurements. Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER. PERIODIC MAINTENANCE Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb. Connectors must be perfectly clean and dry. If any scratches or cracks are present, replace the electrode. Rinse off any salt deposits with water. Probe Maintenance Refill the reference chamber with fresh electrolyte (HI 7082). Allow the electrode to stand upright for 1 hour. Follow the Storage Procedure above. CLEANING PROCEDURE · Wine deposits Soak in Hanna HI 70635 cleaning solution for 15 minutes · Wine stains Soak in Hanna HI 70636 cleaning solution for 15 minutes IMPORTANT: After performing any of the cleaning procedures, rinse the electrode thoroughly with distilled water, refill the reference chamber with fresh electrolyte and soak the electrode in HI 70300 Storage Solution for at least 1 hour before taking measurements. In most situations, 0.8 ppm molecular SO2 during bulk storage and at bottling will provide you with adequate protection from oxidation and bacterial action. This includes prevention of malolactic bacteria as well. It is important to remember that the amount of free SO2 in the wine depends on three things: how much is added, how much was present before the addition and how much of your addition promptly becomes bound. The level at which molecular SO2 can be detected by the human senses is about 2.0 ppm. This is also the level which is needed for maximum protection of your wine. This is particularly true in the case of sweet, and most notably, botrytised wines. The Hanna HI 84100 offers the possibility to test free or total SO2 in all the wines including the red ones, that are difficult to test with manual methods because the color changes are hardly seen. 4 17 FUSE REPLACEMENT To change the fuse follow next steps: · Disconnect the power cord from the rear panel of the instrument. · Pull out the fuse holder located near the power cord connector. · Replace the fuse with a similar one. · Push the fuse holder with the fuse in the appropriate place. SPECIFICATIONS Range Resolution Accuracy Method Principle Sample volume ORP Electrode Pump debit Stirring speed Environment Power supply Dimensions Weight REQUIRED REAGENTS Code HI 84100-50 HI 84100-51 HI 84100-52 HI 84100-53 HI 84100-54 HI 84100-55 0 to 400 ppm of SO2 1 ppm 5% of reading Ripper titrimetric method Equivalence point redox titration 50 mL HI 3148B (included) 0.5 mL/min 1500 rpm 0 to 50 °C (32 to 122 °F); max 95% RH non-condensing 220V/50Hz; 10VA 208 x 214 x 163 mm (8.2 x 8.4 x 6.4") (with beaker) 2200 g (77.6 oz.) Description Titrant (for Free & Total SO2) Alkaline Reagent (for Total SO2) Acid Reagent (for Total SO2) Acid Reagent (for Free SO2) Stabilizer (for Free & Total SO2) Standard (for Free & Total SO2) Quantity/test 5 mL 5 mL 5 mL 1 packet 50 mL ELECTRODE CONDITIONING & MAINTENANCE PREPARATION PROCEDURE Remove the protective cap of the ORP electrode (HI 3148B). DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal with electrodes. They will disappear when rinsed with water. During transport, tiny bubbles of air may form inside the glass bulb affecting proper functi ...