Instruction Manual HI 9146 Portable Waterproof Microprocessor Dissolved Oxygen Meter www.hannainst.com Dear Customer, Thank you for choosing a Hanna Product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for a correct use of the instrument, as well as a precise idea of its versatility. If you need more technical information, do not hesitate to e-mail us at tech@hannainst.com This meter is in compliance with directives. PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer. The meter is supplied complete with: · HI 76407/4F DO probe with 4 m cable and protective sleeve for HI9146-04 · HI 76407/10F DO probe with 10 m cable & protective sleeve for HI9146-10 · 2 membranes (HI 76407A) with O-rings · protective cap · 30 mL electrolyte solution (HI 7041S) · 4 x 1.5V AA batteries · instruction manual · rugged carrying case Note: Save all packing material until you are sure that the instrument functions correctly. Any defective items must be returned in the original packing with the supplied accessories. TABLE OF CONTENTS Preliminary Examination ............................. 3 General Description ..................................... 3 Functional Description - Probe .................... 4 Functional Description - Meter..................... 5 Specifications ............................................. 6 Probe Preparation ....................................... 7 Calibration ................................................... 8 Measurement ............................................ 10 Altitude Compensation .............................. 12 Salinity Compensation .............................. 13 Probe & Membrane Maintenance .............. 14 Battery Replacement ................................ 16 Accessories .............................................. 17 Warranty ................................................... 18 CE Declaration of Conformity .................... 19 2 GENERAL DESCRIPTION The Hanna HI 9146 is a water-resistant, microprocessor-based, auto-calibrating Dissolved Oxygen meter with ATC. It has been developed for dissolved oxygen and temperature measurement in water and wastewater as well as other applications such as fish farming. Dissolved oxygen is indicated in hundredths of parts per million (ppm=mg/L) or in % of saturation . The temperature range is indicated in centigrade from 0 to 50°C with 0.1°C resolution. The ppm and the % saturation are both compensated for changes in solubility of oxygen in water and for permeability of the membrane as well as the temperature effect. 3 Salinity compensation in water allows determination of mg/L of dissolved oxygen in salty waters and the altitude compensation readjusts for the altitude variance. Four 1.5V AA alkaline batteries provide power and make the instrument completely portable. HI 9146 is also designed to be used with a battery recharger or a 12 Vdc power supply. A 12 Vdc input jack is incorporated into the housing. A microprocessor ensures an accurate and rapid calibration and measurement. The case rugged and water-resistant for maximum protection in the field as well as in the laboratory. The dissolved oxygen probe has a membrane covering the polarographic sensor elements and a built-in thermistor for temperature measurement and compensation. The thin permeable membrane isolates the sensor elements from the testing solution, but allows oxygen to enter. When a voltage is applied across the sensor, oxygen that passes through the membrane causes a current to flow from which the oxygen concentration is determined. FUNCTIONAL DESCRIPTION - PROBE 1. D.O. Probe 2. Protective Cap 3. Watertight Shielded Cable 4. P o l y p r o p y l e n e Probe Body 5. Temperature Sensor 6. O-Ring Seal 7. AgCl Anode (sensor element) 8. Platinum Cathode (sensor element) 9. Oxygen Permeable PTFE Membrane 10. Membrane Protective Cap FUNCTIONAL DESCRIPTION - METER 1) 2) 3) 4) 5) 6) 7) 8) 9) 10) 19) 20) 3 1 4 6 7 8 4 9 5 6 7 8 9 2 10 21) 22) 23) 24) Battery Compartment Probe Connector Liquid Crystal Display ON/OFF key CAL key (to enter/exit calibration mode) FACTOR key (to select altitude F1 and salinity F2) UP and DOWN arrow keys (to select F1 and F2 levels) HOLD key (to freeze displayed value) RANGE key (to select ppm or % of saturation) Power socket for 12 Vdc adapter LOW BAT indicator SAMPLE indicator (to indicate measurement mode) F1 indicator (altitude factor) F2 indicator (salinity factor) % or ppm mode indicator Temperature and factor display 5 4 SPECIFICATIONS 0.00 to 45.00 mg/L O2 0.0 to 300 %O2 0.0 to 50.0 °C Resolution 0.01 mg/L O2 0.1 %O2 0.1 °C Accuracy ±1.5% Full Scale mg/L O2 (@25°C/77°F) ±1.5% Full Scale %O2 ±0.5 °C Typical EMC ±0.3 mg/L O2 / ±3.5 %O2 Deviation ±0.5 °C Calibration Automatic in saturated air Temperature Automatic from 0 to 50°C Compensation (32 to 122°F) Altitude 0 to 4 km Compensation 100 m resolution Salinity 0 to 80 g/L Compensation 1 g/L resolution Environment 0 to 50°C (32 to 122°F) RH 100% Power supply · 4x1.5V AA batteries; 200 hours of continuous use; auto-off after 4 hours. · 12 Vdc adapter Dimensions 196 x 80 x 60 mm (7.7 x 3.1 x 2.4") Weight 500 g (1.1 lb.) Range PROBE PREPARATION All probes are shipped dry by Hanna Instruments. To hydrate the probe and prepare it for use, connect it to the meter and proceed as follows. Shipping 1 Remove the red and black plascap tic cap which is for shipping purposes and can be discarded. 2 Wet the sensor by soaking the black bottom 2½ cm (1") of the probe in electrolyte (HI 7041S) for 5 red minutes. 3 Take a membrane (HI 76407A supplied with the meter) and Make FIRST sure that the rub- SECOND INSERT O'RING ber O-ring sits FILL properly inside the membrane THIRD cap. TAP 4 Rinse the membrane with electrolyte while shaking it gently. Refill THEN SCREW with clean electroBACK ON lyte. 5 Gently tap the sides of the membrane cap with your finger tip to ensure that no air bubbles remain trapped. To avoid damaging to the membrane, do not tap the membrane directly on the bottom. 6 With the sensor facing down, screw the cap clockwise. Some electrolyte will overflow. When not in use, protect the membrane by placing the protective cap on it. 6 7 CALIBRATION · PROBE POLARIZATION The probe is under polarization with a fixed voltage of approximately 800 mV. Probe polarization is essential for stable measurements with the same recurring degree of accuracy. With the probe properly polarized, oxygen is continually "consumed" by passing through the sensitive diaphragm and dissolving in the electrolyte solution contained inside the probe. If this operation is interrupted, the electrolyte solution continues to be enriched with oxygen until it reaches an equilibrium with the surrounding solution. Whenever measurements are taken with a non-polarized probe, the oxygen level indicated is that of the test solution as well as any oxygen present in the electrolyte solution. This reading is obviously incorrect. The Hanna oxygen meter shown here automatically polarize the probe when they are switched on. Calibration is simple and is recommended every time the meter is switched on. · Make sure the probe is ready for measurement (see page 5), i.e. the membrane is filled with electrolyte and probe is connected to the meter and properly polarized. · Switch the meter on. · "COND" appears on the display to inform you that ON/OFF the probe is in auto-conditioning (automatic polarization) mode. · Once "COND" disappears the probe is polarized and instrument can be calibrated. · For an accurate calibration, it is recommended to wait an additional 5 or 10 minutes to ensure optimum conditioning of the probe. Remove the protective cap. · · Press CAL. "----" appears on the larger LCD together with "CAL" to indicate that the instrument is in calibration mode. The instrument will automatically standardize itself to the actual saturation value. After approx. 1 minute it will show "100%" on the LCD and a small "SAMPLE" to indicate that the calibration is complete. CAL CAL SAMPLE % °C · Press FACTOR and ensure F1 and F2 are set to the appropriate altitude and salinity values (pages 10-11). Notes: · The instrument must be calibrated whenever the probe, membrane or the electrolyte is changed. · To exit the calibration mode during calibration, press CAL. · The display may be switched from readings in % saturation to mg/ CAL L without recalibration, by simply pressing RANGE. RANGE 8 9 MEASUREMENT Make sure the meter has been calibrated and the protective cap has been removed. Immerse the tip of the probe in the sample to be tested and ensure that the temperature sensor is also immersed. To display values in % saturation, press RANGE. RANGE If the sample contains significant salinity or if the measurement is taken at a higher altitude than sea level, the read-out values must be corrected (by taking into account the lower degree of oxygen solubility in such conditions - see below). Set the altitude and/or the salinity before calibration and taking mg/L measurements. The meter will automatically compensate for these factors. For accurate dissolved oxygen measurements, a water movement of at least 30 cm (12")/sec is required. This is to ensure that oxygen depleted on the membrane surface is constantly replenished. A moving stream will provide adequate circulation. During field measurements, this condition may be obtained by manually stirring the probe. Accurate readings are not possible while the liquid is stationary. During laboratory measurements, the use of a magnetic stirrer to ensure a certain agitation of the fluid is recommended. This way, any errors due to the presence of air bubbles on the membrane surface are minimized. For an accurate measurement, allow sufficient time for thermal equilibrium between the probe and the measurement sample (a few minutes if the temperature difference is several degrees). % O2 SATURATION READINGS The % O2 reading provides the rate of oxygen saturation with reference to 100.0% at sea level. TEMPERATURE READINGS The lower part of the display will show the measured temperature in Celsius degrees. SAMPLE % °C Allow the probe to reach thermal equilibrium with the sample before taking any measurement. The greater the difference between the ambient temperature and the temperature of the sample, the longer it will take the probe to acclimatized itself to the sample. mg/L READINGS The mg/L readings make it possible to read the concentration of the dissolved oxygen directly in ppm. 10 11 ALTITUDE COMPENSATION SALINITY COMPENSATION Press FACTOR and "F1" will be displayed. SAMPLE FACTOR ppm F1 Use the UP and the DOWN keys to set the altitude from 0 and 4.0 km (4000 m), in steps of 0.1 km (100 m). or Press FACTOR twice and "F2" will be displayed. Press UP and DOWN to set the salinity between 0 and 80 g/L. Press FACTOR again to display the temperature. FACTOR SAMPLE ppm F2 or The table provides an idea of the error that can be introduced at different altitudes and the quantity to be subtracted to correct the reading. Altitude above Sea Level (in meters) °C 0 300 600 900 1200 1500 1800 2100 2400 2700 3000 3300 3600 4000 14.1 13.6 13.2 13.3 12.9 12.4 12.7 12.2 11.9 12.0 11.6 11.2 11.4 11.0 10.6 10.9 10.5 10.2 10.4 10.1 9.7 9.9 9.6 9.3 9.7 9.2 8.9 9.2 8.7 8.6 8.8 8.5 8.2 8.4 8.1 7.8 8.1 7.8 7.5 7.8 7.5 7.3 7.5 7.3 7.0 7.2 7.0 6.8 7.1 6.8 6.6 6.9 6.6 6.4 6.6 6.3 6.1 6.4 6.2 5.9 6.2 6.0 5.8 6.1 5.8 5.6 5.9 5.7 5.5 5.7 5.5 5.3 5.6 5.4 5.2 5.4 5.2 5.0 12.7 12.0 11.4 10.8 10.3 9.8 9.4 9.0 8.6 8.3 7.9 7.7 7.3 7.0 6.8 6.5 6.4 6.2 5.9 5.7 5.6 5.4 5.3 5.1 5.0 4.9 12.3 11.6 11.0 10.4 9.9 9.5 9.1 8.7 8.3 8.0 7.7 7.3 7.1 6.8 6.6 6.3 6.1 6.0 5.7 5.6 5.4 5.2 5.1 4.9 4.8 4.7 11.8 11.2 10.6 10.1 9.6 9.2 8.8 8.3 8.0 7.7 7.4 7.1 6.8 6.6 6.3 6.1 5.9 5.8 5.5 5.4 5.2 5.0 4.9 4.8 4.6 4.5 10.9 10.3 9.8 9.3 8.9 8.5 8.1 7.8 7.5 7.2 6.9 6.6 6.3 6.1 5.9 5.7 5.5 5.4 5.2 5.0 4.8 4.7 4.6 4.4 4.3 4.2 10.2 9.6 9.1 8.6 8.2 7.8 7.5 7.2 6.9 6.6 6.3 6.0 5.8 5.7 5.4 5.2 5.1 4.9 4.8 4.6 4.5 4.3 4.3 4.1 4.0 3.9 9.4 8.9 8.5 8.0 7.6 7.3 7.0 6.6 6.4 6.1 5.9 5.6 5.5 5.2 5.0 4.9 4.7 4.6 4.5 4.3 4.2 4.0 4.0 3.8 3.7 3.6 8.7 8.2 7.8 7.4 7.1 6.8 6.4 6.2 5.9 5.7 5.5 5.3 5.1 4.8 4.7 4.6 4.4 4.2 4.1 4.0 3.9 3.7 3.7 3.5 3.5 3.4 8.1 7.7 7.3 6.9 6.5 6.3 6.0 5.7 5.5 5.3 5.1 4.9 4.7 4.5 4.3 4.2 4.1 3.9 3.8 3.7 3.6 3.5 3.4 3.3 3.2 3.1 7.6 7.1 6.7 6.4 6.1 5.8 5.6 5.3 5.1 4.9 4.7 4.5 4.4 4.2 4.0 3.9 3.8 3.7 3.5 3.5 3.3 3.2 3.1 3.1 2.9 2.9 6.6 6.3 6.0 5.7 5.4 5.1 4.9 4.7 4.5 4.3 4.1 4.0 3.8 3.7 3.6 3.5 3.3 3.2 3.1 3.0 2.9 2.9 2.8 2.7 2.6 2.6 0 14.6 2 13.8 4 13.1 6 12.4 8 11.8 10 11.3 12 10.8 14 10.3 16 9.9 18 9.5 20 9.1 22 8.7 24 8.4 26 8.1 28 7.8 30 7.5 32 7.3 34 7.1 36 6.8 38 6.6 40 6.4 42 6.3 44 6.1 46 ...